around 2 weeks1. blockage of fallopian tubes. This makes it harder for the egg to get fertilized by the sperm. Fallopian tube blockage also makes it harder for the egg to travel into the uterus. Even if the egg did manage to get fertilized it would probably lead to an ectopic pregnancy that would cause even more damage to the mother.2. Premature ovarian failure. This is when your ovaries stop functioning correctly because there is not enough estrogen to stimulate the release of FSH and LH. It could also mean that the ovaries do not have enough eggs to release during ovulation. 3. Genetic disorder is another reason for IVF. This is when you or your partner has a genetic disorder that you do not want your offspring to inherit. the person doing the IVF will take the fertilized egg and check it to see if there are any genetic abnormalities. It is not 100% accurate. Sometimes they cannot detect abnormalities until later in the pregnancy. If there are no genetic abnormalities they then transfer the fertilized egg to the uterus.c) 1. multiple births is a problem. IVF allow people to implant multiple embryos in the uterus since not every implanted embryo is guaranteed to survive. However, if multiple implanted embryos do survive it could cause multiple implantations which result in multiple births that tend to be low birth weight and premature. 2. miscarriage. The embryo just doesn’t implant itself in the endometrial lining. 3. Ovarian hyperstimulation syndrome. This happens when you receive too much of any fertility hormone. Having too much of a fertility hormone leads to swollen and painful ovaries. Along with swollen and painful ovaries there are some other symptoms present like abdominal pain, nausea, and vomiting. If pregnancy occurs then the symptoms can last much longer. d) Ovarian testing, seman analysis, infectious disease testing, mock embryo transfer, and a uterine cavity examination are all done before IVF.e) Ovulation induction is when the doctors administer synthetic hormones in order to do various things like : to stimulate your ovaries to release more than one egg during ovulation, help the eggs mature faster, prevent premature ovulation, or prepare the endometrial lining of the uterus for implantation . This is done one to two weeks before retrieval and depending on what you need you might receive a combination of these treatments. The doctor typically performs two tests one to see how the follicles are maturing called a vaginal ultrasound and blood test to see the impact of the synthetic hormones in order to determine when the eggs are ready to be collected. Egg retrieval is then done between 34 to 36 hours after your last synthetic hormone injection and before ovulation. In order to do this procedure the doctors will sedate you and give you pain medication. They will then try to find the follicles either surgically through the abdomen or through the vagina using an ultrasound. To retrieve the egg from the follicle they will use a needle that has a vacuum attached to it gather the mature egg or eggs. The mature eggs are then placed in a medium that is nutritional for the egg and then they are incubated. After the incubation the healthy eggs are then mixed with sperm.f) One fertilization method is incubation. This is when the egg and sperm are mixed together and incubated overnight. Another method is intracytoplasmic sperm injection. This is when the sperm is directly injected into the cytoplasm of the mature egg. This is typically done when the quality or amount of seman is not high. One uncommon method is assisted hatching. This is when the doctor makes a little crack or hole in the zona pellucida in order to help the embryo to implant in the endometrial lining since the zona pellucida prevents implantation. g) The five factor are: the mothers age, the number of days the embryo has developed before being implanted in the uterus( the longer it has developed the better), the number of times you have given birth (more births equals more success), what caused you to have trouble getting pregnant, and what you drink, do, and take on a daily basis (lifestyle).